{"id":105,"date":"2024-11-25T19:23:55","date_gmt":"2024-11-25T19:23:55","guid":{"rendered":"https:\/\/onlineacademiccommunity.uvic.ca\/thechristielab\/?page_id=105"},"modified":"2026-03-24T21:35:19","modified_gmt":"2026-03-24T21:35:19","slug":"molecular","status":"publish","type":"page","link":"https:\/\/onlineacademiccommunity.uvic.ca\/christielab\/molecular\/","title":{"rendered":"Molecular"},"content":{"rendered":"

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m<\/span>olecular<\/span><\/h1>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”-30px|auto||auto|false|false” custom_padding=”||||false|false” locked=”off” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][et_pb_blurb image_icon_background_color=”RGBA(255,255,255,0)” image=”https:\/\/onlineacademiccommunity.uvic.ca\/christielab\/wp-content\/uploads\/sites\/9131\/2024\/12\/Jon_3_SNPs-Rat-JT2-18APN21-3-Mar-20-2019-x100k-crop-full-e1659663226105.png” image_icon_width_tablet=”” image_icon_width_phone=”80px” image_icon_width_last_edited=”on|phone” _builder_version=”4.27.4″ _module_preset=”ac876d58-433b-4b66-ac71-5bd8692a2165″ header_font_size_tablet=”” header_font_size_phone=”16px” header_font_size_last_edited=”on|desktop” body_font_size_last_edited=”off|desktop” border_color_all_image=”#000000″ image_max_width_tablet=”” image_max_width_phone=”80px” image_max_width_last_edited=”on|phone” locked=”off” global_colors_info=”{}” use_background_color_gradient__hover=”off” use_background_color_gradient__hover_enabled=”off” background_color_gradient_start__hover=”#2b87da” background_color_gradient_start__hover_enabled=”#2b87da” background_color_gradient_end__hover=”#29c4a9″ background_color_gradient_end__hover_enabled=”#29c4a9″ background_color_gradient_type__hover=”linear” background_color_gradient_type__hover_enabled=”linear” background_color_gradient_direction__hover=”180deg” background_color_gradient_direction__hover_enabled=”180deg” background_color_gradient_direction_radial__hover=”center” background_color_gradient_direction_radial__hover_enabled=”center” background_color_gradient_start_position__hover=”0%” background_color_gradient_start_position__hover_enabled=”0%” background_color_gradient_end_position__hover=”100%” background_color_gradient_end_position__hover_enabled=”100%” background_color_gradient_overlays_image__hover=”off” background_color_gradient_overlays_image__hover_enabled=”off” parallax__hover=”off” parallax__hover_enabled=”off” parallax_method__hover=”on” parallax_method__hover_enabled=”on” background_size__hover=”cover” background_size__hover_enabled=”cover” background_position__hover=”center” background_position__hover_enabled=”center” background_repeat__hover=”no-repeat” background_repeat__hover_enabled=”no-repeat” background_blend__hover=”normal” background_blend__hover_enabled=”normal” allow_player_pause__hover=”off” allow_player_pause__hover_enabled=”off” background_video_pause_outside_viewport__hover=”on” background_video_pause_outside_viewport__hover_enabled=”on” box_shadow_style__hover=”none” box_shadow_style__hover_enabled=”none” box_shadow_color__hover=”rgba(0,0,0,0.3)” box_shadow_color__hover_enabled=”rgba(0,0,0,0.3)” background_color_gradient_stops__hover=”#2b87da 0%|#29c4a9 100%”]<\/p>\n

Whole Homogenate Sample Generation<\/strong><\/h2>\n

View our written protocol<\/a>.\u00a0<\/span><\/p>\n

[\/et_pb_blurb][et_pb_blurb image_icon_background_color=”RGBA(255,255,255,0)” image=”https:\/\/onlineacademiccommunity.uvic.ca\/christielab\/wp-content\/uploads\/sites\/9131\/2025\/06\/Screenshot-2025-06-13-105936-1.png” content_max_width=”1100px” image_icon_width_tablet=”” image_icon_width_phone=”80px” image_icon_width_last_edited=”on|phone” _builder_version=”4.27.4″ _module_preset=”ac876d58-433b-4b66-ac71-5bd8692a2165″ link_option_url=”https:\/\/www.youtube.com\/watch?v=L4lB05ryxmk&list=PLxP-mzqzVMYxeu9sQwJh9KPnnvQi3kTRR&index=1″ header_font_size_tablet=”” header_font_size_phone=”16px” header_font_size_last_edited=”on|desktop” body_font_size_last_edited=”off|desktop” border_color_all_image=”#000000″ image_max_width_tablet=”” image_max_width_phone=”80px” image_max_width_last_edited=”on|phone” locked=”off” global_colors_info=”{}” use_background_color_gradient__hover=”off” use_background_color_gradient__hover_enabled=”off” background_color_gradient_start__hover=”#2b87da” background_color_gradient_start__hover_enabled=”#2b87da” background_color_gradient_end__hover=”#29c4a9″ background_color_gradient_end__hover_enabled=”#29c4a9″ background_color_gradient_type__hover=”linear” background_color_gradient_type__hover_enabled=”linear” background_color_gradient_direction__hover=”180deg” background_color_gradient_direction__hover_enabled=”180deg” background_color_gradient_direction_radial__hover=”center” background_color_gradient_direction_radial__hover_enabled=”center” background_color_gradient_start_position__hover=”0%” background_color_gradient_start_position__hover_enabled=”0%” background_color_gradient_end_position__hover=”100%” background_color_gradient_end_position__hover_enabled=”100%” background_color_gradient_overlays_image__hover=”off” background_color_gradient_overlays_image__hover_enabled=”off” parallax__hover=”off” parallax__hover_enabled=”off” parallax_method__hover=”on” parallax_method__hover_enabled=”on” background_size__hover=”cover” background_size__hover_enabled=”cover” background_position__hover=”center” background_position__hover_enabled=”center” background_repeat__hover=”no-repeat” background_repeat__hover_enabled=”no-repeat” background_blend__hover=”normal” background_blend__hover_enabled=”normal” allow_player_pause__hover=”off” allow_player_pause__hover_enabled=”off” background_video_pause_outside_viewport__hover=”on” background_video_pause_outside_viewport__hover_enabled=”on” box_shadow_style__hover=”none” box_shadow_style__hover_enabled=”none” box_shadow_color__hover=”rgba(0,0,0,0.3)” box_shadow_color__hover_enabled=”rgba(0,0,0,0.3)” background_color_gradient_stops__hover=”#2b87da 0%|#29c4a9 100%”]<\/p>\n

2. Gel Casting Protocol for Western Blotting<\/strong><\/h2>\n

This video covers how to cast gels for western blotting. View our grapical protocol<\/a>.\u00a0<\/span><\/p>\n

[\/et_pb_blurb][et_pb_blurb image_icon_background_color=”RGBA(255,255,255,0)” image=”https:\/\/onlineacademiccommunity.uvic.ca\/thechristielab\/wp-content\/uploads\/sites\/9131\/2024\/12\/Jon_3_SNPs-Rat-JT2-18APN21-3-Mar-20-2019-x100k-crop-full-e1659663226105.png” content_max_width=”1100px” image_icon_width_tablet=”” image_icon_width_phone=”80px” image_icon_width_last_edited=”on|phone” _builder_version=”4.27.4″ _module_preset=”ac876d58-433b-4b66-ac71-5bd8692a2165″ link_option_url=”https:\/\/www.youtube.com\/watch?v=pwHM_HGOXfQ&list=PLxP-mzqzVMYxeu9sQwJh9KPnnvQi3kTRR&index=3″ header_font_size_tablet=”” header_font_size_phone=”16px” header_font_size_last_edited=”on|desktop” body_font_size_last_edited=”off|desktop” border_color_all_image=”#000000″ image_max_width_tablet=”” image_max_width_phone=”80px” image_max_width_last_edited=”on|phone” locked=”off” global_colors_info=”{}” use_background_color_gradient__hover=”off” use_background_color_gradient__hover_enabled=”off” background_color_gradient_start__hover=”#2b87da” background_color_gradient_start__hover_enabled=”#2b87da” background_color_gradient_end__hover=”#29c4a9″ background_color_gradient_end__hover_enabled=”#29c4a9″ background_color_gradient_type__hover=”linear” background_color_gradient_type__hover_enabled=”linear” background_color_gradient_direction__hover=”180deg” background_color_gradient_direction__hover_enabled=”180deg” background_color_gradient_direction_radial__hover=”center” background_color_gradient_direction_radial__hover_enabled=”center” background_color_gradient_start_position__hover=”0%” background_color_gradient_start_position__hover_enabled=”0%” background_color_gradient_end_position__hover=”100%” background_color_gradient_end_position__hover_enabled=”100%” background_color_gradient_overlays_image__hover=”off” background_color_gradient_overlays_image__hover_enabled=”off” parallax__hover=”off” parallax__hover_enabled=”off” parallax_method__hover=”on” parallax_method__hover_enabled=”on” background_size__hover=”cover” background_size__hover_enabled=”cover” background_position__hover=”center” background_position__hover_enabled=”center” background_repeat__hover=”no-repeat” background_repeat__hover_enabled=”no-repeat” background_blend__hover=”normal” background_blend__hover_enabled=”normal” allow_player_pause__hover=”off” allow_player_pause__hover_enabled=”off” background_video_pause_outside_viewport__hover=”on” background_video_pause_outside_viewport__hover_enabled=”on” box_shadow_style__hover=”none” box_shadow_style__hover_enabled=”none” box_shadow_color__hover=”rgba(0,0,0,0.3)” box_shadow_color__hover_enabled=”rgba(0,0,0,0.3)” background_color_gradient_stops__hover=”#2b87da 0%|#29c4a9 100%”]<\/p>\n

4. Gel Electrophoresis Protocol for Western Blotting<\/strong><\/h2>\n

This video will show how to perform gel electrophoresis for western blotting. View our graphical protocol<\/a>.<\/span><\/p>\n

[\/et_pb_blurb][et_pb_blurb image_icon_background_color=”RGBA(255,255,255,0)” image=”https:\/\/onlineacademiccommunity.uvic.ca\/thechristielab\/wp-content\/uploads\/sites\/9131\/2024\/12\/Jon_3_SNPs-Rat-JT2-18APN21-3-Mar-20-2019-x100k-crop-full-e1659663226105.png” content_max_width=”1100px” image_icon_width_tablet=”” image_icon_width_phone=”80px” image_icon_width_last_edited=”on|phone” _builder_version=”4.27.4″ _module_preset=”ac876d58-433b-4b66-ac71-5bd8692a2165″ link_option_url=”https:\/\/www.youtube.com\/watch?v=kJnI6wIoiJU&list=PLxP-mzqzVMYxeu9sQwJh9KPnnvQi3kTRR&index=5″ header_font_size_tablet=”” header_font_size_phone=”16px” header_font_size_last_edited=”on|desktop” body_font_size_last_edited=”off|desktop” border_color_all_image=”#000000″ image_max_width_tablet=”” image_max_width_phone=”80px” image_max_width_last_edited=”on|phone” locked=”off” global_colors_info=”{}” use_background_color_gradient__hover=”off” use_background_color_gradient__hover_enabled=”off” background_color_gradient_start__hover=”#2b87da” background_color_gradient_start__hover_enabled=”#2b87da” background_color_gradient_end__hover=”#29c4a9″ background_color_gradient_end__hover_enabled=”#29c4a9″ background_color_gradient_type__hover=”linear” background_color_gradient_type__hover_enabled=”linear” background_color_gradient_direction__hover=”180deg” background_color_gradient_direction__hover_enabled=”180deg” background_color_gradient_direction_radial__hover=”center” background_color_gradient_direction_radial__hover_enabled=”center” background_color_gradient_start_position__hover=”0%” background_color_gradient_start_position__hover_enabled=”0%” background_color_gradient_end_position__hover=”100%” background_color_gradient_end_position__hover_enabled=”100%” background_color_gradient_overlays_image__hover=”off” background_color_gradient_overlays_image__hover_enabled=”off” parallax__hover=”off” parallax__hover_enabled=”off” parallax_method__hover=”on” parallax_method__hover_enabled=”on” background_size__hover=”cover” background_size__hover_enabled=”cover” background_position__hover=”center” background_position__hover_enabled=”center” background_repeat__hover=”no-repeat” background_repeat__hover_enabled=”no-repeat” background_blend__hover=”normal” background_blend__hover_enabled=”normal” allow_player_pause__hover=”off” allow_player_pause__hover_enabled=”off” background_video_pause_outside_viewport__hover=”on” background_video_pause_outside_viewport__hover_enabled=”on” box_shadow_style__hover=”none” box_shadow_style__hover_enabled=”none” box_shadow_color__hover=”rgba(0,0,0,0.3)” box_shadow_color__hover_enabled=”rgba(0,0,0,0.3)” background_color_gradient_stops__hover=”#2b87da 0%|#29c4a9 100%”]<\/p>\n

6. Primary Antibody Protocol for Western Blotting<\/strong><\/h2>\n

This video will cover how to use primary antibodies for western blotting. View our graphical protocol<\/a>.<\/span><\/p>\n

[\/et_pb_blurb][et_pb_blurb image_icon_background_color=”RGBA(255,255,255,0)” image=”https:\/\/onlineacademiccommunity.uvic.ca\/thechristielab\/wp-content\/uploads\/sites\/9131\/2024\/12\/Jon_3_SNPs-Rat-JT2-18APN21-3-Mar-20-2019-x100k-crop-full-e1659663226105.png” content_max_width=”1100px” image_icon_width_tablet=”” image_icon_width_phone=”80px” image_icon_width_last_edited=”on|phone” _builder_version=”4.27.4″ _module_preset=”ac876d58-433b-4b66-ac71-5bd8692a2165″ link_option_url=”https:\/\/www.youtube.com\/watch?v=w2VmV9Y0OAw&list=PLxP-mzqzVMYxeu9sQwJh9KPnnvQi3kTRR&index=7″ header_font_size_tablet=”” header_font_size_phone=”16px” header_font_size_last_edited=”on|desktop” body_font_size_last_edited=”off|desktop” border_color_all_image=”#000000″ image_max_width_tablet=”” image_max_width_phone=”80px” image_max_width_last_edited=”on|phone” locked=”off” global_colors_info=”{}” use_background_color_gradient__hover=”off” use_background_color_gradient__hover_enabled=”off” background_color_gradient_start__hover=”#2b87da” background_color_gradient_start__hover_enabled=”#2b87da” background_color_gradient_end__hover=”#29c4a9″ background_color_gradient_end__hover_enabled=”#29c4a9″ background_color_gradient_type__hover=”linear” background_color_gradient_type__hover_enabled=”linear” background_color_gradient_direction__hover=”180deg” background_color_gradient_direction__hover_enabled=”180deg” background_color_gradient_direction_radial__hover=”center” background_color_gradient_direction_radial__hover_enabled=”center” background_color_gradient_start_position__hover=”0%” background_color_gradient_start_position__hover_enabled=”0%” background_color_gradient_end_position__hover=”100%” background_color_gradient_end_position__hover_enabled=”100%” background_color_gradient_overlays_image__hover=”off” background_color_gradient_overlays_image__hover_enabled=”off” parallax__hover=”off” parallax__hover_enabled=”off” parallax_method__hover=”on” parallax_method__hover_enabled=”on” background_size__hover=”cover” background_size__hover_enabled=”cover” background_position__hover=”center” background_position__hover_enabled=”center” background_repeat__hover=”no-repeat” background_repeat__hover_enabled=”no-repeat” background_blend__hover=”normal” background_blend__hover_enabled=”normal” allow_player_pause__hover=”off” allow_player_pause__hover_enabled=”off” background_video_pause_outside_viewport__hover=”on” background_video_pause_outside_viewport__hover_enabled=”on” box_shadow_style__hover=”none” box_shadow_style__hover_enabled=”none” box_shadow_color__hover=”rgba(0,0,0,0.3)” box_shadow_color__hover_enabled=”rgba(0,0,0,0.3)” background_color_gradient_stops__hover=”#2b87da 0%|#29c4a9 100%”]<\/p>\n

8. Imaging Protocol for Western Blotting<\/strong><\/h2>\n

This video will cover how to image a western blot. View our graphical protocol<\/a>.\u00a0<\/span><\/p>\n

[\/et_pb_blurb][et_pb_blurb image_icon_background_color=”RGBA(255,255,255,0)” image=”https:\/\/onlineacademiccommunity.uvic.ca\/christielab\/wp-content\/uploads\/sites\/9131\/2024\/12\/Jon_3_SNPs-Rat-JT2-18APN21-3-Mar-20-2019-x100k-crop-full-e1659663226105.png” content_max_width=”1100px” image_icon_width_phone=”80px” image_icon_width_last_edited=”off|desktop” _builder_version=”4.27.4″ _module_preset=”ac876d58-433b-4b66-ac71-5bd8692a2165″ link_option_url=”https:\/\/onlineacademiccommunity.uvic.ca\/christielab\/wp-content\/uploads\/sites\/9131\/2025\/07\/Synaptoneurosome_flowchart-scaled.png” header_font_size_tablet=”” header_font_size_phone=”16px” header_font_size_last_edited=”on|desktop” body_font_size_last_edited=”off|desktop” border_color_all_image=”#000000″ image_max_width_tablet=”” image_max_width_phone=”80px” image_max_width_last_edited=”on|phone” locked=”off” global_colors_info=”{}” use_background_color_gradient__hover=”off” use_background_color_gradient__hover_enabled=”off” background_color_gradient_start__hover=”#2b87da” background_color_gradient_start__hover_enabled=”#2b87da” background_color_gradient_end__hover=”#29c4a9″ background_color_gradient_end__hover_enabled=”#29c4a9″ background_color_gradient_type__hover=”linear” background_color_gradient_type__hover_enabled=”linear” background_color_gradient_direction__hover=”180deg” background_color_gradient_direction__hover_enabled=”180deg” background_color_gradient_direction_radial__hover=”center” background_color_gradient_direction_radial__hover_enabled=”center” background_color_gradient_start_position__hover=”0%” background_color_gradient_start_position__hover_enabled=”0%” background_color_gradient_end_position__hover=”100%” background_color_gradient_end_position__hover_enabled=”100%” background_color_gradient_overlays_image__hover=”off” background_color_gradient_overlays_image__hover_enabled=”off” parallax__hover=”off” parallax__hover_enabled=”off” parallax_method__hover=”on” parallax_method__hover_enabled=”on” background_size__hover=”cover” background_size__hover_enabled=”cover” background_position__hover=”center” background_position__hover_enabled=”center” background_repeat__hover=”no-repeat” background_repeat__hover_enabled=”no-repeat” background_blend__hover=”normal” background_blend__hover_enabled=”normal” allow_player_pause__hover=”off” allow_player_pause__hover_enabled=”off” background_video_pause_outside_viewport__hover=”on” background_video_pause_outside_viewport__hover_enabled=”on” box_shadow_style__hover=”none” box_shadow_style__hover_enabled=”none” box_shadow_color__hover=”rgba(0,0,0,0.3)” box_shadow_color__hover_enabled=”rgba(0,0,0,0.3)” background_color_gradient_stops__hover=”#2b87da 0%|#29c4a9 100%”]<\/p>\n

Synaptoneurosomes<\/strong><\/h2>\n

Click here to view our flow chart.<\/p>\n

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How to use the Nanodrop<\/strong><\/h2>\n

Click here to view our video<\/a>.<\/p>\n

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1. Bradford Assay Protocol for Western Blotting<\/strong><\/h2>\n

View our grapical protocol<\/a>.\u00a0<\/span><\/p>\n

[\/et_pb_blurb][et_pb_blurb image_icon_background_color=”RGBA(255,255,255,0)” image=”https:\/\/onlineacademiccommunity.uvic.ca\/thechristielab\/wp-content\/uploads\/sites\/9131\/2024\/12\/Jon_3_SNPs-Rat-JT2-18APN21-3-Mar-20-2019-x100k-crop-full-e1659663226105.png” content_max_width=”1100px” image_icon_width_tablet=”” image_icon_width_phone=”80px” image_icon_width_last_edited=”on|phone” _builder_version=”4.27.4″ _module_preset=”ac876d58-433b-4b66-ac71-5bd8692a2165″ background_color=”#FFFFFF” link_option_url=”https:\/\/www.youtube.com\/watch?v=73wvwZ20iTg&list=PLxP-mzqzVMYxeu9sQwJh9KPnnvQi3kTRR&index=2″ header_font_size_tablet=”” header_font_size_phone=”16px” header_font_size_last_edited=”on|desktop” body_font_size_last_edited=”off|desktop” border_color_all_image=”#000000″ image_max_width_tablet=”” image_max_width_phone=”80px” image_max_width_last_edited=”on|phone” locked=”off” global_colors_info=”{}” use_background_color_gradient__hover=”off” use_background_color_gradient__hover_enabled=”off” background_color_gradient_start__hover=”#2b87da” background_color_gradient_start__hover_enabled=”#2b87da” background_color_gradient_end__hover=”#29c4a9″ background_color_gradient_end__hover_enabled=”#29c4a9″ background_color_gradient_type__hover=”linear” background_color_gradient_type__hover_enabled=”linear” background_color_gradient_direction__hover=”180deg” background_color_gradient_direction__hover_enabled=”180deg” background_color_gradient_direction_radial__hover=”center” background_color_gradient_direction_radial__hover_enabled=”center” background_color_gradient_start_position__hover=”0%” background_color_gradient_start_position__hover_enabled=”0%” background_color_gradient_end_position__hover=”100%” background_color_gradient_end_position__hover_enabled=”100%” background_color_gradient_overlays_image__hover=”off” background_color_gradient_overlays_image__hover_enabled=”off” parallax__hover=”off” parallax__hover_enabled=”off” parallax_method__hover=”on” parallax_method__hover_enabled=”on” background_size__hover=”cover” background_size__hover_enabled=”cover” background_position__hover=”center” background_position__hover_enabled=”center” background_repeat__hover=”no-repeat” background_repeat__hover_enabled=”no-repeat” background_blend__hover=”normal” background_blend__hover_enabled=”normal” allow_player_pause__hover=”off” allow_player_pause__hover_enabled=”off” background_video_pause_outside_viewport__hover=”on” background_video_pause_outside_viewport__hover_enabled=”on” box_shadow_style__hover=”none” box_shadow_style__hover_enabled=”none” box_shadow_color__hover=”rgba(0,0,0,0.3)” box_shadow_color__hover_enabled=”rgba(0,0,0,0.3)” background_color_gradient_stops__hover=”#2b87da 0%|#29c4a9 100%”]<\/p>\n

3. Sample Preparation Protocol for Western Blotting<\/strong><\/h2>\n

This video will cover how to prepare samples for western blotting.<\/span><\/p>\n

[\/et_pb_blurb][et_pb_blurb image_icon_background_color=”RGBA(255,255,255,0)” image=”https:\/\/onlineacademiccommunity.uvic.ca\/thechristielab\/wp-content\/uploads\/sites\/9131\/2024\/12\/Jon_3_SNPs-Rat-JT2-18APN21-3-Mar-20-2019-x100k-crop-full-e1659663226105.png” content_max_width=”1100px” image_icon_width_tablet=”” image_icon_width_phone=”80px” image_icon_width_last_edited=”on|phone” _builder_version=”4.27.4″ _module_preset=”ac876d58-433b-4b66-ac71-5bd8692a2165″ link_option_url=”https:\/\/www.youtube.com\/watch?v=AGh1xJ6oyj8&list=PLxP-mzqzVMYxeu9sQwJh9KPnnvQi3kTRR&index=4″ header_font_size_tablet=”” header_font_size_phone=”16px” header_font_size_last_edited=”on|desktop” body_font_size_last_edited=”off|desktop” border_color_all_image=”#000000″ image_max_width_tablet=”” image_max_width_phone=”80px” image_max_width_last_edited=”on|phone” locked=”off” global_colors_info=”{}” use_background_color_gradient__hover=”off” use_background_color_gradient__hover_enabled=”off” background_color_gradient_start__hover=”#2b87da” background_color_gradient_start__hover_enabled=”#2b87da” background_color_gradient_end__hover=”#29c4a9″ background_color_gradient_end__hover_enabled=”#29c4a9″ background_color_gradient_type__hover=”linear” background_color_gradient_type__hover_enabled=”linear” background_color_gradient_direction__hover=”180deg” background_color_gradient_direction__hover_enabled=”180deg” background_color_gradient_direction_radial__hover=”center” background_color_gradient_direction_radial__hover_enabled=”center” background_color_gradient_start_position__hover=”0%” background_color_gradient_start_position__hover_enabled=”0%” background_color_gradient_end_position__hover=”100%” background_color_gradient_end_position__hover_enabled=”100%” background_color_gradient_overlays_image__hover=”off” background_color_gradient_overlays_image__hover_enabled=”off” parallax__hover=”off” parallax__hover_enabled=”off” parallax_method__hover=”on” parallax_method__hover_enabled=”on” background_size__hover=”cover” background_size__hover_enabled=”cover” background_position__hover=”center” background_position__hover_enabled=”center” background_repeat__hover=”no-repeat” background_repeat__hover_enabled=”no-repeat” background_blend__hover=”normal” background_blend__hover_enabled=”normal” allow_player_pause__hover=”off” allow_player_pause__hover_enabled=”off” background_video_pause_outside_viewport__hover=”on” background_video_pause_outside_viewport__hover_enabled=”on” box_shadow_style__hover=”none” box_shadow_style__hover_enabled=”none” box_shadow_color__hover=”rgba(0,0,0,0.3)” box_shadow_color__hover_enabled=”rgba(0,0,0,0.3)” background_color_gradient_stops__hover=”#2b87da 0%|#29c4a9 100%”]<\/p>\n

5. Transfer Protocol for Western Blotting<\/span><\/h2>\n

This video will cover how to perform the transfer protocol for western blotting. View our graphicaol protocol<\/a>.<\/span><\/p>\n

[\/et_pb_blurb][et_pb_blurb image_icon_background_color=”RGBA(255,255,255,0)” image=”https:\/\/onlineacademiccommunity.uvic.ca\/thechristielab\/wp-content\/uploads\/sites\/9131\/2024\/12\/Jon_3_SNPs-Rat-JT2-18APN21-3-Mar-20-2019-x100k-crop-full-e1659663226105.png” content_max_width=”1100px” image_icon_width_tablet=”” image_icon_width_phone=”80px” image_icon_width_last_edited=”on|phone” _builder_version=”4.27.4″ _module_preset=”ac876d58-433b-4b66-ac71-5bd8692a2165″ link_option_url=”https:\/\/www.youtube.com\/watch?v=7jPzyfeJKok&list=PLxP-mzqzVMYxeu9sQwJh9KPnnvQi3kTRR&index=6″ header_font_size_tablet=”” header_font_size_phone=”16px” header_font_size_last_edited=”on|desktop” body_font_size_last_edited=”off|desktop” border_color_all_image=”#000000″ image_max_width_tablet=”” image_max_width_phone=”80px” image_max_width_last_edited=”on|phone” locked=”off” global_colors_info=”{}” use_background_color_gradient__hover=”off” use_background_color_gradient__hover_enabled=”off” background_color_gradient_start__hover=”#2b87da” background_color_gradient_start__hover_enabled=”#2b87da” background_color_gradient_end__hover=”#29c4a9″ background_color_gradient_end__hover_enabled=”#29c4a9″ background_color_gradient_type__hover=”linear” background_color_gradient_type__hover_enabled=”linear” background_color_gradient_direction__hover=”180deg” background_color_gradient_direction__hover_enabled=”180deg” background_color_gradient_direction_radial__hover=”center” background_color_gradient_direction_radial__hover_enabled=”center” background_color_gradient_start_position__hover=”0%” background_color_gradient_start_position__hover_enabled=”0%” background_color_gradient_end_position__hover=”100%” background_color_gradient_end_position__hover_enabled=”100%” background_color_gradient_overlays_image__hover=”off” background_color_gradient_overlays_image__hover_enabled=”off” parallax__hover=”off” parallax__hover_enabled=”off” parallax_method__hover=”on” parallax_method__hover_enabled=”on” background_size__hover=”cover” background_size__hover_enabled=”cover” background_position__hover=”center” background_position__hover_enabled=”center” background_repeat__hover=”no-repeat” background_repeat__hover_enabled=”no-repeat” background_blend__hover=”normal” background_blend__hover_enabled=”normal” allow_player_pause__hover=”off” allow_player_pause__hover_enabled=”off” background_video_pause_outside_viewport__hover=”on” background_video_pause_outside_viewport__hover_enabled=”on” box_shadow_style__hover=”none” box_shadow_style__hover_enabled=”none” box_shadow_color__hover=”rgba(0,0,0,0.3)” box_shadow_color__hover_enabled=”rgba(0,0,0,0.3)” background_color_gradient_stops__hover=”#2b87da 0%|#29c4a9 100%”]<\/p>\n

7. Secondary Antibody Protocol for Western Blotting<\/strong><\/h2>\n

This video will cover how to use secondary antibodies for western blotting. View our graphical protocol.<\/a><\/span><\/p>\n

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9. Ponceau Stain Protocol for Western Blotting<\/strong><\/h2>\n

This video will cover how to perform a Ponceau stain for western blotting. View our graphical protocol<\/a>.\u00a0<\/span><\/p>\n

[\/et_pb_blurb][et_pb_blurb image_icon_background_color=”RGBA(255,255,255,0)” image=”https:\/\/onlineacademiccommunity.uvic.ca\/thechristielab\/wp-content\/uploads\/sites\/9131\/2024\/12\/Jon_3_SNPs-Rat-JT2-18APN21-3-Mar-20-2019-x100k-crop-full-e1659663226105.png” content_max_width=”1100px” image_icon_width_tablet=”” image_icon_width_phone=”80px” image_icon_width_last_edited=”on|phone” _builder_version=”4.27.4″ _module_preset=”ac876d58-433b-4b66-ac71-5bd8692a2165″ link_option_url=”https:\/\/onlineacademiccommunity.uvic.ca\/christielab\/wp-content\/uploads\/sites\/9131\/2025\/07\/Western-Blot-Written-Protocol.pdf” header_font_size_tablet=”” header_font_size_phone=”16px” header_font_size_last_edited=”on|desktop” body_font_size_last_edited=”off|desktop” border_color_all_image=”#000000″ image_max_width_tablet=”” image_max_width_phone=”80px” image_max_width_last_edited=”on|phone” locked=”off” global_colors_info=”{}” use_background_color_gradient__hover=”off” use_background_color_gradient__hover_enabled=”off” background_color_gradient_start__hover=”#2b87da” background_color_gradient_start__hover_enabled=”#2b87da” background_color_gradient_end__hover=”#29c4a9″ background_color_gradient_end__hover_enabled=”#29c4a9″ background_color_gradient_type__hover=”linear” background_color_gradient_type__hover_enabled=”linear” background_color_gradient_direction__hover=”180deg” background_color_gradient_direction__hover_enabled=”180deg” background_color_gradient_direction_radial__hover=”center” background_color_gradient_direction_radial__hover_enabled=”center” background_color_gradient_start_position__hover=”0%” background_color_gradient_start_position__hover_enabled=”0%” background_color_gradient_end_position__hover=”100%” background_color_gradient_end_position__hover_enabled=”100%” background_color_gradient_overlays_image__hover=”off” background_color_gradient_overlays_image__hover_enabled=”off” parallax__hover=”off” parallax__hover_enabled=”off” parallax_method__hover=”on” parallax_method__hover_enabled=”on” background_size__hover=”cover” background_size__hover_enabled=”cover” background_position__hover=”center” background_position__hover_enabled=”center” background_repeat__hover=”no-repeat” background_repeat__hover_enabled=”no-repeat” background_blend__hover=”normal” background_blend__hover_enabled=”normal” allow_player_pause__hover=”off” allow_player_pause__hover_enabled=”off” background_video_pause_outside_viewport__hover=”on” background_video_pause_outside_viewport__hover_enabled=”on” box_shadow_style__hover=”none” box_shadow_style__hover_enabled=”none” box_shadow_color__hover=”rgba(0,0,0,0.3)” box_shadow_color__hover_enabled=”rgba(0,0,0,0.3)” background_color_gradient_stops__hover=”#2b87da 0%|#29c4a9 100%”]<\/p>\n

Western Blot Written Protocol<\/strong><\/h2>\n

Written protocol for western blots with all steps included.\u00a0<\/span><\/p>\n

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qPCR<\/span><\/h1>\n

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Telomere Length Quantification<\/span><\/strong><\/h2>\n

Find our\u00a0<\/span>video protocol\u00a0<\/span><\/a>and\u00a0<\/span>written protocol<\/a>\u00a0here!<\/span><\/p>\n

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molecularWhole Homogenate Sample Generation View our written protocol.\u00a02. Gel Casting Protocol for Western Blotting This video covers how to cast gels for western blotting. View our grapical protocol.\u00a04. Gel Electrophoresis […]<\/p>\n","protected":false},"author":20367,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-105","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/onlineacademiccommunity.uvic.ca\/christielab\/wp-json\/wp\/v2\/pages\/105","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/onlineacademiccommunity.uvic.ca\/christielab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/onlineacademiccommunity.uvic.ca\/christielab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/onlineacademiccommunity.uvic.ca\/christielab\/wp-json\/wp\/v2\/users\/20367"}],"replies":[{"embeddable":true,"href":"https:\/\/onlineacademiccommunity.uvic.ca\/christielab\/wp-json\/wp\/v2\/comments?post=105"}],"version-history":[{"count":45,"href":"https:\/\/onlineacademiccommunity.uvic.ca\/christielab\/wp-json\/wp\/v2\/pages\/105\/revisions"}],"predecessor-version":[{"id":3817,"href":"https:\/\/onlineacademiccommunity.uvic.ca\/christielab\/wp-json\/wp\/v2\/pages\/105\/revisions\/3817"}],"wp:attachment":[{"href":"https:\/\/onlineacademiccommunity.uvic.ca\/christielab\/wp-json\/wp\/v2\/media?parent=105"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}