Regular aCSF
Click here to view our video on making regular artificial cerebrospinal fluid.
Cutting Solutions
Coming soon: how to make cutting solutions for electrophysiology!
Preparation Steps
Steps to follow at the beginning of your experiment.
Dissection
How to dissect brain from a rat or mouse.
Sectioning with a Ted Pella 1000 Vibratome
How to section a brain using a Ted Pella 1000 Vibratome.
Sectioning with a Precisionary Compresstome VF-310-0Z
How to section a brain into slices using a Precisionary Instruments compresstome!
Electrode Pulling
Things to consider when pulling pipettes for recording electrodes.
Making Stimulation Electrodes
Coming soon: How to make stimulation electrodes!
Getting Started with pCLAMP
Coming soon: How to use pCLAMP!
Introduction to Field Recordings
Coming soon: Field Recordings for electrophysiology!
Data Analysis
Coming soon: How to analyze electrophysiology data!
Statistics with Python
Video covers a workflow for how to organize electrophysiology data for use in Python. Python code used in this video can be downloaded here.
Whole Cell Patch Clamp
Introduction to Whole Cell
Click here to view our full video on whole cell patch clamping.
Chapter 2 - Solution Preparation
Click here to view our video on solution prep for whole cell patch clamping.
Chapter 3 - Slice Preparation
Click here to view our video on slice preparation for whole cell patch clamping.
Chapter 4 - Rig Setup
Click here to view our video on rig setup for whole cell patch clamping.
Chapter 5 - Finding a Cell
Click here to view our video on finding a cell for whole cell patch clamping.
Chapter 6 - The Giga-Ohm Seal
Click here to view our video on the Giga-Ohm Seal for whole cell patch clamping.
Chapter 7 - Breaking the Patch
Click here to view our video on breaking the seal for whole cell patch clamping.