Get familiarized with the main components of the Olympus FluoView FV1000 software with this video tutorial series. The following contains step-by-step instructions and video walkthroughs on how to acquire an XYZ image series at 10X magnification.
Permission to use the confocal microscope must be granted by Dr. Christie personally.
Take care to turn the instruments on and off in the following order:
On
1. Mercury Lamp
2. [Key] Lasers 488 and 561
3. “Red Laser”
4. Microscope Control Box
5. [Key] Confocal Unit
6. Software
Off
6. Software
5. [Key] Confocal Unit
4. Microscope Control Box
3. “Red Laser”
2. [Key] Lasers 488 and 561
1. Mercury Lamp
Set the conditions and locate the region of interest under the epifluorescent lamp.
Learn how to use the Live View window to optimize your image.
Acquire an XYZ image series under 10X magnification.