Immunohistochemistry

Antibodies-101

Antibodies 101: How to prolong the use of antibodies in the lab.

C – Fos

Click here to view a video on our immunofluorescence staining protocol for C-Fos. View our written and graphical protocols.

Iba1

This video covers the step by step protocol for Iba1 immunohistochemistry to target and visualize microglia. View our graphical and written protocols. 

CB1 Staining

Click here to view the CB1 Receptor immunohistochemical staining protocol.

BrdU

Click here to view a video that demonstrates our immunohistochemical staining protocol for targeting BrdU positive cells in rat brain tissue. View our graphical protocol.

Ki-67

Graphical protocol on our immunofluorescence staining for Ki-67 

Neuro-D

DCX Immunohistochemistry

DCX Immunohistochemistry on Free Floating Brain Sections

Immunohistochemistry Analysis

ImageJ

Image processing and Analysis in Java. View our graphical and written protocols.

Dendritic Spine Analysis & 3D Neuron Reconstruction

Watch our video to see how we use MBF’s Neurolucida 360 software for dendritic spine analysis and 3D neuron reconstruction.

Optical Density: Calibration with Step Tablet

View part 1 of our series on measuring optical density using ImageJ here!

Optical Density: Setting Scale on FIJI

View part 3 of our series on measuring optical density using ImageJ here!

Optical Density: Running Macro in FIJI

View part 2 of our series on measuring optical density using ImageJ here!