Histology and Immunohistochemistry

Iba1

This video covers the step by step protocol for Iba1 immunohistochemistry to target and visualize microglia.

BrdU

 Click here to view a video that demonstrates our immunohistochemical staining protocol for targeting BrdU positive cells in rat brain tissue. See our graphical protocol.

C-Fos

Click here to view a video on our immunofluorescence staining protocol for C-Fos. See our written and graphical protocols.

Ki-67

A video and graphical protocol on our immunofluorescence staining protocol for Ki-67 will be posted shortly – come back soon!

Sectioning with a Leica VT1000-S Vibratome

Click here to view our video on sectioning a mouse brain using a Leica VT1000-S Vibratome.

Sectioning with a Leica VT1200 Vibratome

Click here to view our video on sectioning a mouse brain using a Leica VT1200 Vibratome.

DiI For Dendritic Spine Labelling

DiI Crystal Placement and Spine Visualization

This video describes how to label neurons within the hippocampus using DiI crystals and how to visualize using confocal microscopy.

Dendritic Spine Analysis & 3D Neuron Reconstruction (Neurolucida 360)

Watch our video to see how we use MBF’s Neurolucida 360 software for dendritic spine analysis and 3D neuron reconstruction.