electrophysiology
In vivo Electrophysiology
Vernier Scale
Click here to watch our video discussing how to use a vernier scale!
In vitro Electrophysiology
Introduction to Whole Cell
Click here to view our full video on whole cell patch clamping.
1. Preparation Steps
Steps to follow at the beginning of your experiment.
3. Slice Preparation
Click here to view our video on solution prep for whole cell patch clamping.
5. Finding a Cell
Click here to view our video on solution prep for whole cell patch clamping.
Electrode Pulling
Things to consider when pulling pipettes for recording electrodes.
2. Solution Preparation
Click here to view our video on solution prep for whole cell patch clamping.
4. Rig Setup
Click here to view our video on solution prep for whole cell patch clamping.
6. The Giga-0hm Seal
Click here to view our video on the Giga-Ohm Seal for whole cell patch clamping.
7. Breaking in
Click here to view our video on breaking the seal for whole cell patch clamping.
Set Up and Optimization
Click here to view our slide show on how to optimize your whole cell set up
In vitro Solutions
Regular aCSF
Click here to view our video on making regular artificial cerebrospinal fluid.