| Factors | Description |
| Electrode Glass | Drummond Scientific Company 100 microliter pipettes cat: 2-000-100 |
| Heat | Affects length and size. Reducing increases tip size and vice versa. |
| Pull | Should be set to 0 when pulling large (1-10micron) pipettes. |
| Velocity | Should be between 10 and 30 for patch pipettes. |
| Time | Increasing the time will produce shorter tips. |
| Pressure | Should be 500 in most cases. |
Neuroscience for Healthier Brains